Doc. RNDr. Petr MLEJNEK, Ph.D.
Telephone:
585632203, 585632202
Workplace:
Ústav normální anatomie
Org. unit:
Lékařská fakulta
Address:
Teoretické ústavy
Room number:
3.061
Profession:
docent
ČLÁNEK
Mlejnek P.
What Is the Significance of Lysosomal-Mediated Resistance to Imatinib?
Cells.
2023.
Mlejnek P.
Direct Interaction between N-Acetylcysteine and Cytotoxic Electrophile-An Overlooked In Vitro Mechanism of Protection.
Antioxidants.
2022.
Mlejnek P., Havlásek J., Pastvová N., Doležel P., Dostálová K.
Lysosomal sequestration of weak base drugs, lysosomal biogenesis, and cell cycle alteration.
BIOMEDICINE & PHARMACOTHERAPY.
2022.
Pastvová N., Doležel P., Mlejnek P.
Heat Shock Protein Inhibitor 17-Allyamino-17-Demethoxygeldanamycin, a Potent Inductor of Apoptosis in Human Glioma Tumor Cell Lines, Is a Weak Substrate for ABCB1 and ABCG2 Transporters.
Pharmaceuticals.
2021.
Pastvová N., Havlásek J., Doležel P., Kikalová K., Študentová H., Zemánková A., Melichar B., Mlejnek P.
Changes in expression of lysosomal membrane proteins in leucocytes of cancer patients treated with tyrosine kinase inhibitors.
CANCER CHEMOTHERAPY AND PHARMACOLOGY.
2021.
Mlejnek P., Doležel P., Kriegová E., Pastvová N.
N-acetylcysteine Can Induce Massive Oxidative Stress, Resulting in Cell Death with Apoptotic Features in Human Leukemia Cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES.
2021.
Mlejnek P., Havlásek J., Pastvová N., Doležel P.
Can image analysis provide evidence that lysosomal sequestration mediates daunorubicin resistance?
CHEMICO-BIOLOGICAL INTERACTIONS.
2020.
Skoupá N., Doležel P., Mlejnek P.
Lysosomal Fusion: An Efficient Mechanism Increasing Their Sequestration Capacity for Weak Base Drugs without Apparent Lysosomal Biogenesis.
Biomolecules.
2020.
Mlejnek P., Kosztyu P., Doležel P., Kimura Y., Čížková K., Růžičková E.
Estimation of ABCB1 concentration in plasma membrane.
JOURNAL OF CELLULAR BIOCHEMISTRY.
2019.
Dostál Z., Kosina P., Mlejnek P., Kikalová K., Modrianský M.
Mifepristone potentiates etoposide toxicity in Hep G2 cells by modulating drug transport.
TOXICOLOGY IN VITRO.
2019.
Mlejnek P., Doležel P., Maier V., Kikalová K., Skoupá N.
N-acetylcysteine dual and antagonistic effect on cadmium cytotoxicity in human leukemia cells.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY.
2019.
Růžičková E., Skoupá N., Doležel P., Smith DA., Mlejnek P.
The Lysosomal Sequestration of Tyrosine Kinase Inhibitors and Drug Resistance.
Biomolecules.
2019.
Skoupá N., Doležel P., Růžičková E., Mlejnek P.
Apoptosis Induced by the Curcumin Analogue EF-24 Is Neither Mediated by Oxidative Stress-Related Mechanisms nor Affected by Expression of Main Drug Transporters ABCB1 and ABCG2 in Human Leukemia Cells.
International Journal of Molecular Sciences (online).
2017.
Růžičková E., Janská R., Doležel P., Mlejnek P.
Clinically relevant interactions of anti-apoptotic Bcl-2 protein inhibitors with ABC transporters.
PHARMAZIE.
2017.
Mlejnek P., Kosztyu P., Doležel P., Růžičková E., Bates SE.
Reversal of ABCB1 mediated efflux by imatinib and nilotinib in cells expressing various transporter levels.
Chemico-Biological Interactions.
2017.
Mlejnek P., Doležel P., Kosztyu P., Václavíková R.
Can the assessment of ABCB1 gene expression predict its function in vitro?
European Journal of Haematology.
2015.
Mlejnek P., Doležel P.
Loss of mitochondrial transmembrane potential and glutathione depletion are not sufficient to account for induction of apoptosis by carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone in human leukemia K562 cells.
Chemico-Biological Interactions.
2015.
Mlejnek P., Doležel P.
N-acetylcysteine prevents the geldanamycin cytotoxicity by forming geldanamycin-N-acetylcysteine adduct.
Chemico-Biological Interactions.
2014.
Kosztyu P., Bukvová R., Doležel P., Mlejnek P.
Resistance to daunorubicin, imatinib, or nilotinib depends on expression levels of ABCB1 and ABCG2 in human leukemia cells.
Chemico-Biological Interactions.
2014.
Kosztyu P., Doležel P., Mlejnek P.
Can P glycoprotein mediate resistance to nilotinib in human leukemia cells?
Pharmacological Research.
2013.
Mlejnek P.
Cytokinin-induced cell death is associated with elevated expression of alternative oxidase in tobacco BY-2 cells.
Protoplasma: an international journal of cell biology.
2013.
Mlejnek P., Doležel P., Frydrych I.
Effects of synthetic A3 adenosine receptor agonists on cell proliferation and viability are receptor independent at micromolar concentrations.
Journal of Physiology and Biochemistry.
2013.
MLEJNEK P., DOLEŽEL P., KOSZTYU P.
P-glycoprotein mediates resistance to A3 adenosine receptor agonist 2-chloro-N6-(3-iodobenzyl)-adenosine-5?-N-methyluronamide in human leukemia cells.
Journal of Cellular Physiology.
2012.
MLEJNEK P., NOVÁK O., DOLEŽEL P.
A non-radioactive assay for precise determination of intracellular levels of imatinib and its main metabolite in Bcr-Abl positive cells.
Talanta.
2011.
KRUMPOCHOVÁ P., Kocúrová A., DOLEŽEL P., MLEJNEK P.
Assay for determination of daunorubicin in cancer cells with multidrug resistance phenotype.
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences.
2011.
MLEJNEK P., DOLEŽEL P., FABER E., KOSZTYU P.
Interactions of N-desmethyl imatinib, an active metabolite of imatinib, with P-glycoprotein in human leukemia cells.
Annals of Hematology (print).
2011.
Doležel P., Koudelková P., Mlejnek P.
Halogenation of N6-benzyladenosine decreases its cytotoxicity in human leukemia cells.
Toxicology in Vitro.
2010.
Mlejnek P., Doležel P.
Induction of apoptosis by A3 adenosine receptor agonist N6-(3-iodobenzyl)-adenosine-5'-N-methylcarboxamide in human leukemia cells: a possible involvement of intracellular mechanism.
Acta Physiologica.
2010.
Mojzíková R., Doležel P., Pavlíček J., Mlejnek P., Pospíšilová D., Divoký V.
Partial glutathione reductase deficiency as a cause of diverse clinical manifestations in a family with unstable hemoglobin (Hemoglobin Haná, beta63(E7) His-Asn).
Blood Cells, Molecules and Diseases.
2010.
Frydrych I., Mlejnek P., Doležel P.
Cyclosporin A sensitises Bcr-Abl positive cells to imatinib mesylate independently of P-glykoprotein expression.
Toxicology in Vitro.
2009.
Horáková K., Mlejnková H., Mlejnek P.
Evaluation of methods for isolation of DNA for polymerase chain reaction (PCR)-based identification of pathogenic bacteria from pure cultures and water samples.
Water Science and Technology.
2008.
Frydrych I., Doležel P., Mlejnek P.
P-glycoprotein overexpression confers resistance to A3 adenosine receptor agonists 2-chloro-N6-(3-iodobenzyl)-adenosine-5′-N-methyluronamide (Cl-IB-MECA) in human leukemia cells.
Purinergic Signalling.
2008.
Frydrych I., Mlejnek P.
Serine protease inhibitors N-alpha-tosyl-L-lysinyl-chloromethylketone (TLCK) and N-tosyl-L-phenylalaninyl-chloromethylketone (TPCK) are potent inhibitors of activated caspase proteases.
Journal of Cellular Biochemistry.
2008.
Frydrych I., Mlejnek P.
Serine protease inhibitors N-alpha-tosyl-L-lysinyl-chloromethylketone (TLCK) and N-tosyl-L-phenylalaninyl-chloromethylketone (TPCK) do not inhibit caspase-3 and caspase-7 processing in cells exposed to pro-apoptotic inducing stimuli.
Journal of Cellular Biochemistry.
2008.
Horáková K., Mlejnková H., Mlejnek P.
Specific detection of Escherichia coli isolated from water samples using polymerase chain reaction targeting four genes: cytochrome bd complex, lactose permease, beta-D-glucuronidase, and beta-D-galactosidase.
Journal of Applied Microbiology.
2008.
Frydrych I., Mlejnek P., Doležel P., Zoumpourlis V., Krumpochová P.
The broad-spectrum caspase inhibitor Boc-Asp-CMK induces cell death in human leukaemia cells.
Toxicology in Vitro.
2008.
Mlejnek P., Frydrych I., Doležel P.
Cyclosporin A potentiates the cytotoxic effects of methyl methanesulphonate in HL-60 and K562 cells.
ATLA - Alternatives to Laboratory Animals.
2007.
Solná R., Doležel P., Mlejnek P., Divoký V.
Modification of phenotype of unstable hemoglobinopathy (Hemoglobin Hana [α2β2 63 (E7) His-Asn)]) by partial glutathione reductase deficiency.
Haematologica: the hematology journal.
2007.
Horáková K., Mlejnková H., Mlejnek P.
Direct detection of bacterial faecal indicators in water samples using PCR.
Water Science and Technology.
2006.
Mlejnek P., Doležel P.
Apoptosis induced by N6-substituted derivatives of adenosine is related to intracellular accumulation of corresponding mononucleotides in HL-60 cells.
Toxicology in Vitro.
2005.
Mlejnek P.
Can application of serine protease inhibitors TPCK and TLCK provide evidence for possible involvement of serine protease Omi/HtrA2 in imatinib mesylate-induced cell death of BCR-ABL-positive human leukemia cells?
Leukemia.
2005.
Mlejnek P., Doležel P., Procházka S.
Intracellular conversion of cytokinin bases into corresponding mononucleotides is related to cell death induction in tobacco BY-2 cells.
Plant Science.
2005.
KNIHA - CELEK
Jonák jr. J., Jonák J., Mlejnek P.
Programovaná buněčná smrt u živočichů a rostlin.
Molekulární biologie a genetika XII (pracovní materiály pro přednáškový kurs).
2006.
PATENT, UŽITNÝ VZOR, PRŮMYSLOVÝ VZOR
Moravcová D., Havlíček L., Kryštof V., Lenobel R., Binarová P., Mlejnek P., Vojtěšek B., Uldrijan S., Schmülling T., Strnad M.
Pyrazolo[4,3-D]pyrimidines, process for their preparation and methods of use.
2012.
Moravcová D., Havlíček L., Kryštof V., Lenobel R., Binarová P., Mlejnek P., Vojtěšek B., Uldrijan S., Schmulling T., Strnad M.
Pyrazolo[4,3-D]pyrimidines, process for their preparation and methods of use.
2010.
Doležal K., Popa I., Zatloukal M., Lenobel R., Hradecká D., Vojtěšek B., Uldrijan S., Mlejnek P., Werbrouck S., Strnad M.
Substitution derivatives of N(6)-benzyladenosine, methods of their preparation, their use for preparation of drugs, cosmetic preparations and growth regulators, pharmaceutical prep., cosmetic prep. and growth regulators containing these comp.
Ústav experimentální botaniky AV ČR.
2007.
Doležal K., Popa I., Zatloukal M., Lenobel R., Hradecká D., Vojtěšek B., Uldrijan S., Werbrouck S., Strnad M., Mlejnek P.
Substitution derivatives of N6-benzyladenosine, methods of their preparation, their use for preparation of drugs, cosmetic preparations and growth regulators, pharmaceutical preparations, cosmetic preparations and growth regulators containing these c.
Ústav experimentální botaniky AV ČR.
2007.
Doležal K., Popa I., Zatloukal M., Lenobel R., Hradecká D., Vojtěšek B., Uldrijan S., Mlejnek P., Werbrouck S., Strnad M.
Substitution derivatives of N6-benzyladenosine, methods of their preparation, their use for preparation of drugs, cosmetic preparations and growth regulators, pharmaceutical preparations, cosmetic preparations and growth regulators containing these c.
Ústav Experimentální Botaniky AV ČR.
2006.
Doležal K., Popa I., Zatloukal M., Lenobel R., Hradecká D., Vojtěšek B., Uldrijan S., Mlejnek P., Werbrouck S., Strnad M.
Substituční deriváty N6-benzyladenosinu, způsob jejich přípravy, jejich použití pro přípravu léčiv, kosmetických přípravků a růstových regulátorů, farmaceutické přípravky, kosmetické přípravky a růstové regulátory tyto sloučeniny obsahující.
Ústav experimentální botaniky AV ČR.
2005.
Doležal K., Popa I., Zatloukal M., Lenobel R., Hradecká D., Vojtěšek B., Uldrijan S., Mlejnek P., Werbrouck S., Strnad M.
Substitution derivatives of N6-benzyladenosine, methods of their preparation, their use for preparation of drugs, cosmetic preparations and growth regulators, pharmaceutical preparations, cosmetic preparations and growth regulators containing.
Ústav experimentální botaniky AVČR.
2005.
ABSTRAKT
Dostál Z., Kosina P., Mlejnek P., Kikalová K., Modrianský M.
Inhibition of ABC transporters by mifepristone is responsible for enhanced etoposide toxicity in Hep G2 cells.
FEBS Open Bio.
2018.
KOSZTYU P., Kocurová A., MLEJNEK P.
Does the extracellular acidosis really increase the activity of P glycoprotein in cancer cells?
Toward toxicity assessment without animals.
2011.
KOSZTYU P., DOLEŽEL P., MLEJNEK P.
Quantitative relationship between the P-glycoprotein expression and the drug resistance in leukemic cells.
Sborník abstrakt.
2011.
Kosztyu P., Doležel P., Mlejnek P.
Kvantitativní vztah mezi expresí P-glykoproteinu a pozorovanou rezistenci u nádorových buněk.
Konference Biotechnologie v medicíně, 27.5. 29.5. 2010, Mlýnhotel Vílanec u Jihlavy, CR.
2010.
Kosztyu P., Doležel P., Mlejnek P.
Modulace exprese P-glykoproteinu technikou RNA interference.
Chemické listy.
2010.
Kosztyu P., Doležel P., Mlejnek P.
Reversal of P-glycoprotein-mediated multidrug resistance by RNA interference.
ATP-Binding Cassette (ABC) Proteins: From Multidrug Resistance to Genetic Diseases, únor 2010.
2010.
Frydrych I., Doležel P., Mlejnek P.
Serine protease inhibitors impair mitochondrial and lysosomal functions in human leukemia cells.
27th Annual Workshop of Scandinavian Society for Cell Toxicology.
2009.
Mlejnek P., Doležel P., Frydrych I.
Cancer cells exhibit enhanced resistance to selective A3 adenosine receptor agonist 2-chloro-N6-(3-iodobenzyl)-adenosine-5′-N-methyluronamide (Cl-IB-MECA) due to overexpression of P-glycoprotein.
ESTIV invitox 2008, Abstract Book.
2008.
Krumpochová P., Doležel P., Mlejnek P.
Contribution of P-glycoprotein overexpression to the development of resistance to daunorubicine in cancer cells: a quantitative approach.
ESTIV invitox 2008, Abstract Book.
2008.
Krumpochová P., Frydrych I., Doležel P., Mlejnek P.
Correlation between intracellular drug level and cell resistance in P-glycoprotein expressing tumor cells.
ATP-Binding Cassette (ABC) proteins: From multidrug resistance to genetic diseases, Abstract Book.
2008.
Mlejnek P., Frydrych I., Doležel P.
Cyklosporin A sensitises Bcr-Abl positive cells to imatinib mesylate independently of P-glykoprotein expression.
ESTIV invitox 2008, Abstract Book.
2008.
Mlejnek P., Doležel P.
Induction of apoptosis by adenosine A3 receptor agonists N6-(3-iodobenzyl)-adenosine-5'-N-methylcarboxamide (IB-MECA) in human leukemia cells: a possible involvement of intracellular mechanism.
Purinergic Signalling.
2008.
Frydrych I., Krumpochová P., Doležel P., Mlejnek P.
Serine protease inhibitors N-α-tosyl-L-lysinyl-chloromethylketone (TLCK) and N-tosyl-L-phenylalaninyl-chloromethylketone (TPCK) exhibit adverse effects on mitochondrial function.
ESTIV invitox 2008, Abstract Book.
2008.
Mlejnek P., Frydrych I., Doležel P.
The indirect methods for assessment of P-glycoprotein function do not provide satisfactory evidence for the actual contribution of the P-glycoprotein mediated efflux to the multidrug resistance phenotype in cancer cells.
ATP-Binding Cassette (ABC) proteins: From multidrug resistance to genetic diseases, Abstract Book.
2008.
Mlejnek P., Frydrych I., Doležel P., Krumpochová P., Novotný J.
A method for direct estimation of intracellular drug concentration.
24th SSCT Annual workshop on in vitro toxicology at Grinda on 7-9 September 2006, Programme and Abstracts.
2006.
Solná R., Doležel P., Mlejnek P., Divoký V.
Antioxidant Status of Red Blood Cells Is a Modifying Factor of Clinical Manifestation of Unstable Hemoglobin Variant Hana [2ß2 63 (E7) His-Asn)].
Blood (print).
2006.
Frydrych I., Mlejnek P.
N6-benzyladenine induces increased expression of adenine phosphoribosyltransferase mRNA in mammalian leukaemia cells.
Purinergic Signalling.
2006.
Frydrych I., Mlejnek P.
N6-Benzyladenine potentiates cytotoxic effect of other bases by induction of increased activity of APRT enzyme.
Laboratorní hematologie 2006.
2006.
Frydrych I., Mlejnek P.
N-α-tosyl-L-lysinyl-chloromethylketone (TLCK) inhibits many apoptotic features in dying K562 cells but it does not prevent cells from death.
24th SSCT Annual workshop on in vitro toxicology at Grinda on 7-9 September 2006, Programme and Abstracts.
2006.
Mlejnek P.
Poskytuje používání fluorescenčních sond rhodaminu 123 a daunorubicinu spolehlivá data o funkci P-glykoproteinu?
XX. Olomoucké hematologické dny s mezinárodní účastí.
2006.
Solná R., Mlejnek P., Doležel P., Pospíšilová D., Indrák K., Divoký V.
Sledování míry oxidačního stresu v erytrocytech u některých vrozených hemolytických anémií.
XX. Olomoucké hematologické dny s mezinárodní účastí.
2006.
Mlejnek P., Doležel P., Frydrych I., Novotný J.
Stanovení intracelulárního obsahu imatinibu u buněčné linie K562.
XX. Olomoucké hematologické dny s mezinárodní účastí.
2006.
Frydrych I., Krumpochová P., Mlejnek P.
Verapamil i cyklosporin A zvyšují účinnost cytotoxických látek u leukemických buněčných linií nezávisle na expresi P-glykoproteinu.
XX. Olomoucké hematologické dny s mezinárodní účastí.
2006.
Krumpochová P., Frydrych I., Mlejnek P.
Vliv inhibitorů P-glykoproteinu na účinnost protinádorových léčiv u buněčné linie K562.
Laboratorní hematologie 2006.
2006.
Mlejnek P., Frydrych I.
Cyclosporin A potentiates cytotoxic effect of methyl methanesulfonate in HL-60 cells.
Programme and Abstract, EST SSCT Joint Conference ?Chemicals, human and Environment?
2005.
Uživatel nepovolil zobrazení dat v Portále UP.